Journal: bioRxiv
Article Title: A newly identified three-domain C-type lectin associated with blood feeding in the tick Ixodes ricinus
doi: 10.64898/2026.01.07.698205
Figure Lengend Snippet: Schematic representation of ligands recognized by all three CRD domains in glycan-array experiments. The ligands are groupped according to a blood group antigen from which they are derived. The symbolic representation of saccharides: yellow circle = D-galactose; yellow square = N -acetyl-D-galactosamine; blue circle = D-glucose; blue square = N -acetyl-D-glucosamine; red rectangle = L-fucose; purple diamond = N -acetylneuraminic acid.
Article Snippet: For the hemagglutination inhibition assay, 25 μL of the recombinant His-CRD1 or His-CRD2 was mixed with 25 μL of serially diluted saccharides (α-L-fucose (L-Fuc), α-D-galactose (D-Gal), N -acetyl-α-D-galactosamine (GalNAc) (Carbosynth Limited), α-L-galactose (L-Gal), α-D-glucose (D-Glc), α-D-mannose (D-Man) (Duchefa Biochemie) dissolved in buffer A. Mixtures were incubated for 15 min at room temperature.
Techniques: Glycoproteomics, Derivative Assay